Strain that contains the plasmid, but no GalT insert. Enzymatic activity is measured by the coupled enzyme activity assay as described previously, with the results shown rein Table I. hydrogenase.68 Eight histidines were replaced by various residues rein this study, including four conserved histidines in the putative transmembrane helices. Substitution https://site-directed-mutagenesis43197.worldblogged.com/27535548/top-site-directed-mutagenesis-geheimnisse