Subsequently, a 200-μl fixation buffer was accustomed to repair the cells for twenty min. Right after washing the cells 3 times with precooled PBS, one× intracellular staining permeabilization wash buffer made up of one% DAPI (blue) was applied to mark the nuclei on the cells. Ten minutes afterwards, the cells were https://pimavanserin-tartrate54321.canariblogs.com/5-tips-about-adr-925-you-can-use-today-41802987